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KMID : 0378119820090010076
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Chungnam Medical Journal
1982 Volume.9 No. 1 p.76 ~ p.82
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Improvement of Method for Detection of Leprosy Bacilli from Patients
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崔大卿/Choi, Tae Kyung
張明雄/朴鼎圭/Chang, Myung Woong/Park, Jeong Kyu
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Abstract
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For the improvement of detection of leprosy bacilli from patient, author´s attention of the bacterial detection ratio have been devoted to compared the skin smear method with the nuclepore filter method from 200 patients who were registrated in Chuagnam Skin Clinic of Korean Leprosy Association. One hundred patients out of 200 registered were positive in bacterial detection, the others were negative.
For the dispersion of bacterial globi in nuclepore filter method, the skin scrapped materials were treated with 0.025% trypsin and 0.002 % tween 80 for 30 minutes at 37°C incubator. The efficiency of bacterial detection between the nuclepore 13 mm diameter of the filter and the 25 mm filter were investigated in the broth cultured M. fortuitum.
The cultured M. fortuitum was transfered 1 ml into each 10 tubes, and then was carried out ten-fold dilution, respectively. The specimen for comparative counting of acid fast bacilli were made on 10-* dilution in using 25 mm diameter of filter and 10-5 dilution in using 13 mm diameter of filter and cultural method.
The results were summarized as follows:
1. The ratio of bacterial detection by the skin smear and nuclepore filter methods from 100 patients who were registrated with bacterial detection positive cases were not observed any difference, in all cases were detected leprosy bacilli with both methods.
2. In 100 patients who were registrated with bacterial detection negative, 19 patients
were detected leprosy bacilli by the skin smear method and 33 patients were detected
leprosy bacilli by the nuclepore filter method. The ratio of bacterial detection by two
methods were observed statistically significant difference. (0.013. Between bacterial indices by skin smear and bacterial numbers of nuclepore filter met-
hods were observed perfect positive corelation.
4. There was close corelation between 25 mm filter and the culture method, however, 13 mm filter was more higher than other two methods.
From the above results, it was suggested that the nuclepore filter method with well digesting specimens with 0.025% trypsin and 0.002% tween 80 would be more accurate and higher detection ratio of M. leprae than skin smear method.
The nuclepore 13mm filter was more efficient to detection of acid fast bacilli than 25 mm filter.
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